Determination of the content of Nandrolone Phenylpropionate Powder

- Sep 22, 2019-

Other carcasses take appropriate amount of this product, accurately weighed, using methanol as solvent, formulated into 2mg solution per 1ml (1) and 0.04mg solution per 1ml (2). Using the method and solution under the content determination, 8 μl was injected into the liquid chromatograph to adjust the sensitivity of the instrument so that the peak height of the main component reached the full scale of the recorder. Then, 8 μl of each of the solutions (1) and (2) were separately taken and injected. Record the chromatogram to 1.5 times the retention time of the principal component peak. The number of impurity peaks in solution (1) shall not exceed 3, and the area of each impurity peak and its sum shall not exceed 1/2 and 3/4 of the main peak area of solution (2), respectively. Take the weight loss of the product, place it in a phosphorus pentoxide dryer, dry it under reduced pressure to constant weight, and reduce the weight by 0.5% (Appendix VIIIL).

Determination of content: determined by high performance liquid chromatography (Appendix VD). Chromatographic conditions and system suitability test, using octadecylsilane bonded silica as a filler, methanol-water (82:18) as mobile phase; detection wavelength was 241nm. The number of theoretical plates should be no less than 2300 according to the Nandrolone phenylpropionate, and the separation of the Nylon peak of phenylpropionate and the internal standard substance should meet the requirements. Preparation of internal standard solution Take about 50mg of testosterone propionate, accurately weighed, placed in a 50ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well, that is.

Determination method: take about 50mg of phenylpropionate reference substance, accurately weighed, placed in a 25ml volumetric flask, add methanol to dissolve and dilute to the mark, shake well; accurately measure the solution and the internal standard solution 5ml each, set the amount of 25ml In the bottle, dilute to the mark with methanol, shake well; take 8μl into the liquid chromatograph, record the chromatogram; take the appropriate amount of the product, the same method, according to the internal standard method to calculate the peak area, that is.