Determined by high performance liquid chromatography (Appendix VD). Chromatographic conditions and system suitability test using octadecylsilane bonded silica as a filler; methanol-water (65:35) as mobile phase; detection wavelength was 254nm. The number of theoretical plates should be no less than 1000 according to the peak of progesterone, and the resolution of the peak of progesterone and the peak of internal standard should meet the requirements. Preparation of the internal standard solution Take about 25 mg of diethylstilbestrol, accurately weighed and placed in a 25 ml volumetric flask.
Dissolve in methanol and dilute to the mark, shake well, that is. Determination method Take about 25mg of this product, accurately weighed, placed in a 25ml volumetric flask, dissolved in methanol and diluted to the mark, shake well; accurately measure the solution and the internal standard solution 5ml each, set in a 25ml volumetric flask, diluted with methanol To the mark, shake well, take 5 μl into the liquid chromatograph, and record the chromatogram.